Journal:

Article Title: Relationships between Colony Morphotypes and Oil Tolerance in Rhodococcus rhodochrous

doi:

Figure Lengend Snippet: Growth of rhodococci on seawater-based medium containing the AF of Arabian light crude oil a

Article Snippet: The results supported and generalized our notion that S-2 EPS protects rough Rhodococcus strains from the toxicity of n -hexadecane. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain % of surviving cells Without EPS With EPS R. coprophilus ATCC 29080 0.1 (±0.2) 1.8 (±0.78) R. erythropolis IFO 15567 0.9 (±1.2) 75.3 (±81.6) R. erythropolis JCM 3201 0.1 (±0.1) 48.6 (±25.4) R. globerulus IFO 14531 0.01 (±0.01) 8.6 (±0.1) R. opacus ATCC 51881 1.1 (±1.0) 18.2 (±1.1) R. rhodochrous ATCC 13808 0 (±0) 72.6 (±6.7) R. zopfii ATCC 51349 0.5 (±0.5) 46.7 (±23.6) Open in a separate window a The EPS concentration used in this test was 100 μg/ml.

Techniques:

Journal:

Article Title: Relationships between Colony Morphotypes and Oil Tolerance in Rhodococcus rhodochrous

doi:

Figure Lengend Snippet: Effect of S-2 EPS on the survival of resting cells of Rhodococcus strains treated with 10% (vol/vol) n -hexadecane a

Article Snippet: The results supported and generalized our notion that S-2 EPS protects rough Rhodococcus strains from the toxicity of n -hexadecane. table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Strain % of surviving cells Without EPS With EPS R. coprophilus ATCC 29080 0.1 (±0.2) 1.8 (±0.78) R. erythropolis IFO 15567 0.9 (±1.2) 75.3 (±81.6) R. erythropolis JCM 3201 0.1 (±0.1) 48.6 (±25.4) R. globerulus IFO 14531 0.01 (±0.01) 8.6 (±0.1) R. opacus ATCC 51881 1.1 (±1.0) 18.2 (±1.1) R. rhodochrous ATCC 13808 0 (±0) 72.6 (±6.7) R. zopfii ATCC 51349 0.5 (±0.5) 46.7 (±23.6) Open in a separate window a The EPS concentration used in this test was 100 μg/ml.

Techniques:

Journal: Scientific Reports

Article Title: Impaired retinoic acid signaling in cerebral cavernous malformations

doi: 10.1038/s41598-023-31905-0

Figure Lengend Snippet: Expression of retinoic acid pathway components is misregulated in in vitro and in vivo models of CCM. (a ) Retinoic acid synthesis and degradation pathway. Synthesis of retinoic acid from Vitamin A (retinol) is dependent on availability of NADPH. In this study, all-trans RA and the Cyp26 inhibitor Talarozole were used to modulate RA levels. ( b ) Comparative expression levels of RA pathway genes in ccm2 mutant zebrafish hearts, pan-endothelial Ccm2 knock-out mouse veins, and siRNA CCM2-depleted HUVECs. Fold changes in expression levels are depicted in circle sizes (grouped by fold changes between 0 and 5, see legend). Downregulated transcripts are represented with blue circles whereas upregulated genes are shown in red circles. Transcripts not represented in the datasets do not contain a circle. Gene names are represented as human orthologs except for genes for which only co-orthologs are known (marked with asterisks). ( c ) Whole-mount in situ hybridization of raldh2 performed on 72 hpf old zebrafish and magnifications of the heart region (see box). Stained valve leaflets are marked by an arrow.

Article Snippet: For treatment, either 0.1 µM Retinoic Acid (#R2625, Sigma) or 1–10 µM Talarozole (14531-10, Hycultec GmbH) was added and controls were supplemented with the same volume of DMSO.

Techniques: Expressing, In Vitro, In Vivo, Mutagenesis, Knock-Out, In Situ Hybridization, Staining

Journal: Scientific Reports

Article Title: Impaired retinoic acid signaling in cerebral cavernous malformations

doi: 10.1038/s41598-023-31905-0

Figure Lengend Snippet: Curative effects upon Talarozole treatment of krit1 mutant zebrafish and retinoic acid treatment of siCCM2 depleted HUVECs. (a ) Treatment scheme for zebrafish from 18 to 48 hpf using Talarozole. Endocardial cell numbers (a readout for CCM-associated cardiac phenotype in zebrafish) were analyzed after treatment in two independent experiments. ( b ) Representative images of zebrafish heart morphologies visualized by the transgene kdrl:EGFP at 48 hpf after treatment. Upper: heart morphology of controls treated with DMSO. Lower: hearts treated with Talarozole. Scale bars: 100 µm. ( c ) Endocardial cell counts at 48 hpf of zebrafish treated with several doses of Talarozole or DMSO (*P < 0.05; ****P < 0.0001 vs. control). Each data point represents one heart. ( d ) Treatment regimen of HUVECS. SiRNA transfection was done for 24 h, followed by treatment with RA 24 h later. Cell morphologies were analyzed after 48 h of incubation and antibody staining. ( e ) Immunohistochemistry of HUVECS for beta-catenin, F-actin, and DAPI. First column: control cells (siCT) with wild-type CCM2 expression. Second column: untreated siCCM2 -depleted HUVECs. Third column: representative image showing siCCM2 HUVECs treated with 100 nM RA. Scale bars: 10 µm.

Article Snippet: For treatment, either 0.1 µM Retinoic Acid (#R2625, Sigma) or 1–10 µM Talarozole (14531-10, Hycultec GmbH) was added and controls were supplemented with the same volume of DMSO.

Techniques: Mutagenesis, Transfection, Incubation, Staining, Immunohistochemistry, Expressing

Journal: Scientific Reports

Article Title: Impaired retinoic acid signaling in cerebral cavernous malformations

doi: 10.1038/s41598-023-31905-0

Figure Lengend Snippet: Assessment of KLF2/4 mRNA expression in treated animal models of CCM. (a ) Expression levels of treated BEC iCcm2 mouse cerebella after curative RA treatment (~ 20 mg/kg/day for 21 days) as mean fold changes determined by qRT-PCR. (ns = P > 0.05; **P < 0.01;***P < 0.001;****P < 0.0001 vs. control). Each data point represents one sample. Error bars show mean with SD. ( b ) Fold changes of KLF2/4 mRNA expression in siCCM2 -transfected HUVECs treated with RA for 48 h compared to untreated cells (*P < 0.05; **P < 0.01). Each data point represents one sample. ( c ) Whole-mount in situ hybridization of klf2a in 48 hpf zebrafish embryos treated with DMSO or Talarozole.

Article Snippet: For treatment, either 0.1 µM Retinoic Acid (#R2625, Sigma) or 1–10 µM Talarozole (14531-10, Hycultec GmbH) was added and controls were supplemented with the same volume of DMSO.

Techniques: Expressing, Quantitative RT-PCR, Transfection, In Situ Hybridization